欧州実験生物学ジャーナル オープンアクセス

抽象的な

develop micro clonal propagation protocol for oxytenanthera abyssinica a rich munro to large scale micro propagation

Adugnaw Admas

In Ethiopia, Oxytenanthera abyssinica A.Rich. Munro   has varies economic  and enviroment importance. However, conventional propagation methods of O. abyssinica are generally inefficient due to their low multiplication rate, time consuming, labor intensive, and too costly.The objective of this study was to develop a protocol for mass micropropagation of  O. abyssinica through seed culture. Murashige and Skoog (MS) medium augmented with  6-Benzylaminopurine  (BAP)  was  used for shoot initiation and multiplication. For in vitro rooting, MS medium supplemented with 3-Indole –butric acid ( IBA)    was used.
In shoot initiation experiment all viable seeds were proliferated in 5-7 days of culturing. In shoot   multiplication at 0.004 g/L   BAP was Sucssefuly shoot multiplied , also best root responding were   found at 0.005 g/l  IBA. 
The present optimized protocol enables for any acters who needs large numbers of  low land bamboo seedling for industery ,small and micro enterprize  and  for reafforestation programms.