K.Swathi*, N. Sunitha
A reverse phase liquid chromatography (RP-HPLC) method has been developed and subsequently validated for the determination of Imiquimod. Separation was achieved with a cosmosil C18, 250 mm × 4.6 mm I.D; particle size 5 μm) Column and pH 4.6 phosphate buffer and Acetonitrile (20:80) v/v as mobile phase at a flow rate of 0.8 mL/min and the Column temperature was maintained at 25°C. UV detection was performed at 244 nm with a run time of 10 min. The method is simple, rapid, and selective. The described method of imiquimod is linear over a range of 5 ng/ mL to 600 ng/mL and with correlation coefficient of 0.992 respectively. The method precision for the determination of assay was below 2.0% RSD. The method enables accurate, precise, and rapid analysis of imiquimod.
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