Ahmad Rafiee Pour, Seyed Saeed Mirzargar, Mehdi Soltani, Hossein Ali Ebrahimzadeh Mousavi and Seyed Ali Mostafavi
The aim of this study were to investigate the antibacterial effects of Cuminum cyminum L. and Rosmarinus officinalis essential oil and extracts against Lactococcus garvieae in laboratory conditions on rainbow trout. The study also aimed to identify the minimum inhibitory concentration (MIC) of Cuminum cyminum L. and Rosmarinus officinalis essential oil against this strain (Lactococcus garvieae). In this experiment, 11 strains of Lactococcus garvieae were collected from different provinces of Iran. In addition, we has been evaluated the antibacterial effects of Cuminum cyminum L. and Rosmarinus officinalis extracts and essential oils. After the laboratory cultured Lactococcus garvieae strains, the different dilutions of Cuminum cyminum L. and Rosmarinus officinalis essential oils were added to the culture medium at three different temperatures (20, 30 and 37 °C). Differences between extracts and essential oil of C. cyminum L. and Rosmarinus officinalis were tested with analysis of variance. Results of this experiment showed that the extracts of Cuminum cyminum L. and Rosmarinus officinalis against Lactococcus garvieae has not effects. The minimum inhibitory concentration of C. cyminum L. essential oil in the culture medium of strains L. garvieae at three different temperatures were effective. From the results of the present study it can be concluded that Cuminum cyminum L. and Rosmarinus officinalis essential oils could be effective for the inhibition of Lactococcus garvieae in rainbow trout.
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